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MessagePosté le: Lun 31 Oct - 06:26 (2016) Répondre en citantRevenir en haut




Heterophilic Antibodies A Problem For All Immunoassays Pdf Download > shorl.com/gugohifapihu

























































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Kilinc AS, Dzoylum A, Uncugil CF, Ycel D. 2002;48:6779. [PubMed]21. Clin Endocrinol. Autoantibody against testosterone in a woman with hypergonadotropic hypogonadism. The magnitude of the effect depends on the concentration of the interfering substance, but not necessarily in a directly proportional way. Falsely lowered FT4 and raised TSH concentrations in a patient with hyperthyroidism and human anti-mouse monoclonal antibodies. False-negative results can occur in two-site assays also, presumably due to HAMA reacting with one of the antibodies and preventing reaction with the analyte.80 Methods that use only one mouse monoclonal antibody in IMA assays are less prone to interference from HAMA.81 The interference caused by anti-animal antibodies can be eliminated by sample pre-treatment or assay redesign.80High-dose Hook EffectIn IMA systems where the analyte concentration range is large, antigen-antibody reactions can go into antigen excess and can result in false-negative results, e.g., ferritin, growth hormone, hCG, prolactin, Tg,89–93 and potentially lead to misdiagnosis.91 In particular, for two-site immunoassays in which both the capture and detection antibody are added simultaneously, free analyte and analyte bound to labelled antibody compete for the limited number of antibody-binding sites and in the presence of higher analyte concentration will decrease rather than increase label bound to the solid phase. Clin Biochem.

Of these analyses, 1.8% (n = 65) of results involving 13 analytes were determined to be heterophile false-positive while another 4.2% (n = 146) of results involving 17 analytes gave false-positives of uncertain etiology that were not restored to within the reference interval by addition of heterophile blocking reagent. Kroll MH, Elin RJ. Clin Chem. [PubMed]44. Approaches to minimizing interference by cross-reacting molecules in immunoassays. Ismail AAA, Walker PL, Barth JH, Lewandowski KC, Jones R, Burr WA. [PubMed]30.

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